基于生物信息数据库探讨雷公藤红素抗糖尿病及其并发症的生物分子机制研究

袁永亮, 李泽运, 杜玥, 张基, 杨志衡, 田鑫

中国药学杂志 ›› 2020, Vol. 55 ›› Issue (4) : 305-311.

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中国药学杂志
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中国药学杂志 ›› 2020, Vol. 55 ›› Issue (4) : 305-311. DOI: 10.11669/cpj.2020.04.007
论著

基于生物信息数据库探讨雷公藤红素抗糖尿病及其并发症的生物分子机制研究

  • 袁永亮, 李泽运, 杜玥, 张基, 杨志衡, 田鑫*
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Bio-molecular Mechanism of Celastrol Anti-diabetes and Its Complications Based on Biological Information Database

  • YUAN Yong-liang, LI Ze-yun, DU Yue, ZHANG Ji, YANG Zhi-heng, TIAN Xin*
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摘要

目的 基于生物信息数据库探讨雷公藤红素抗糖尿病及其并发症的生物分子机制研究。方法 从生物信息数据库中获取雷公藤红素的相关联基因信息,通过Veen图软件寻找雷公藤红素和糖尿病(diabetes mellitus)交集基因,采用STRING软件构建蛋白-蛋白互作网络,运用DAVID软件和KOBAS软件进行相关KEGG通路识别,进一步用R语言编程软件构建了雷公藤红素抗糖尿病的“化合物-靶基因-通路-疾病”相关生物分子机制网络。结果 雷公藤红素有50个基因与糖尿病相关,涉及到的通路有11条(P<0.001),主要影响糖尿病并发症中的AGE-RAGE信号通路,其机制是通过抑制NF-κB调节 AGE-RAGE-NF-κB轴,减少下游炎症效应因子产生,进而减轻胰岛素抵抗和器官纤维化损伤。结论 雷公藤红素能够通过调节AGE-RAGE-NF-κB轴相关信号通路,改善糖尿病及其并发症的相关症状。雷公藤红素有望成为治疗糖尿病及其并发症的潜在药物。

Abstract

OBJECTIVE To investigate the biomolecular anti-diabetes mechanism and its complications of celastrol based on bioinformatics database. METHODS The related genes of celastrol were obtained from the bioinformatics database, the cross-related genes of celastrol and diabetes mellitus were searched by Veen diagram software, the protein-protein interaction network was constructed by STRING software, the related KEGG pathway was identified by the DAVID software and KOBAS software, and the molecular network of anti-diabetes "compound-target gene-pathway-disease" was further constructed by R program software. RESULTS There were 50 genes related to diabetes in celastrol, and 11 related pathways (P<0.001), which mainly affected the AGE-RAGE signaling pathway in diabetic complications. The mechanism was to regulate AGE-RAGE-NF-κB axis by inhibiting NF-κB, which reduced the production of downstream inflammatory effectors, insulin resistance and fibrotic damage. CONCLUSION Celastrol can improve the symptoms of diabetes and its complications by regulating the AGE-RAGE-NF-κB axis-related signaling pathway, which is expected to be a potential drug for the treatment of diabetes and its complications.

关键词

雷公藤红素 / 糖尿病 / 生物信息 / 分子机制 / 通路

Key words

celastrol / diabetes mellitus / bioinformation / molecular mechanism / pathway

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袁永亮, 李泽运, 杜玥, 张基, 杨志衡, 田鑫. 基于生物信息数据库探讨雷公藤红素抗糖尿病及其并发症的生物分子机制研究[J]. 中国药学杂志, 2020, 55(4): 305-311 https://doi.org/10.11669/cpj.2020.04.007
YUAN Yong-liang, LI Ze-yun, DU Yue, ZHANG Ji, YANG Zhi-heng, TIAN Xin. Bio-molecular Mechanism of Celastrol Anti-diabetes and Its Complications Based on Biological Information Database[J]. Chinese Pharmaceutical Journal, 2020, 55(4): 305-311 https://doi.org/10.11669/cpj.2020.04.007
中图分类号： R965   

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基金

国家自然科学基金项目资助(U1504831,81703598,81603287,81903874)
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